Analysis of protein expression spectrum related to esophageal cancer family history by ClinProt system

Objective To seek for the serum protein biomarkers related to esophageal cancer hereditary susceptibility of esophageal cancer patients in high esophageal cancer incident area.Methods the subjects were 66 esophageal cancer patients from high esophageal cancer incident area.Among them,there were 28 patients with esophageal cancer family history and 38 patients without esophageal cancer family history.All patients' serums were collected.By Clinprot magnetic bead purification,matrix-assisted laser desorption/ionization time of flightmass spectrometry(MALDI-TOF-MS) to analyze corresponding protein finger print,We applied ClinProTools bioinformatic methods to study serum protein spectrum,and to compare serum protein expression spectrum in family history positive esophageal cancer patients with that in family history negative esophageal cancer patients and to screen the expected protein related to the hereditary susceptibility of esophageal cancer.Sigma serum was used in every sample to repeat the detection.Results We used mean coefficient of variability(CV) to assess repeatability,and mean CV was 0.232.In the range of relative molecular mass between 800 and 10 000 Da,101 proteins were detected in family history positive esophageal cancer patients and family history negative esophageal cancer patients.Five proteins with the strongest combination and subgroup ability group were chosen as category variable to establish category predictive model,which mass charge ratio(M/Z) was 5 248.24,5 905.05,2 023.12,2 953.07 and 4 467.16.We tested it and found the differential protein expression have no difference in family history positive esophageal cancer patients and family history negative esophageal cancer patients(P0.05).We used GA(heredity algorithm) algorithm to select differential protein,recognition rate in family history positive esophageal cancer patients and family history negative esophageal cancer patients was 82.62%.Accurate rate was 69.23% in family history positive esophageal cancer patients,and 96% in family history negative esophageal cancer patients.Predict ability was 61.89%.We used SNN(heredity algorithm) to select differential protein.Recognition rate and accurate rate in family history positive esophageal cancer patients and family history negative esophageal cancer patients were both 100%.Prediction ability was 53.25%.Conclusion The reactive proteins had no relationship with esophageal cancer family history.To seek family based etiology of esophageal cancer,we need to examine and analyze other factors in a larger scale.Perhaps there was no significant correlation between the response protein with esophageal family history after esophageal mucosal epithelium cells became cancerous.