Production and characterization of cellulases and xylanases of Cellulosimicrobium cellulans grown in pretreated and extracted bagasse and minimal nutrient medium M9

Bagasse, the main coproduct in the sugar industry, has been shown to be the sole carbon source for liquid-state fermentation to produce hydrolytic enzymes (cellulases and xylanases) using Cellulosimicrobium cellulans in flask during 3 days and the enzymatic extracts obtained at different time intervals were analysed. The degradation of bagasse indicate the lignocellulolytic capacity of C. cellulans, 26% decrease in hemicellulose content at 96 h, while 10% decrease in cellulose. This accord to that xylanases fairly predominated over cellulases. A rapid rise in the activity of cellulases and xylanases was observed in the early stages of incubation (within the first 24 h). The optimum pH for these enzymes by C. cellulans in liquid cultures was between 5.0 and 5.6 and the optimum temperature was 50 °C. Thermal stability of the enzymes was appreciable up to 45 °C, pH stability at pH 5.0.