H3N8 subtype equine influenza recombinant virus-like particle vaccine as well as preparation method and application thereof

The invention discloses an H3N8 subtype equine influenza recombinant virus-like particle vaccine as well as a preparation method and an application thereof. The vaccine in the invention contains recombinant virus-like particle protein which is obtained by expressing nucleotide sequences as shown in SEQ ID NO.1 and SEQ ID NO.2 through an insect/baculovirus expression system, a pharmaceutically acceptable carrier, an adjuvant or an excipient. The recombinant virus-like particle has a morphological structure similar with that of a natural virus particle, and has good immunogenicity; the vaccine prepared by the recombinant virus-like particle can induce a mouse to generate good protective immune response after immunizing the mouse, and is free of any risk of live virus; and meanwhile, naturally infected persons and immune persons can be distinguished, so that epidemiological investigation is not affected, and therefore, the H3N8 subtype equine influenza recombinant virus-like particle vaccine disclosed by the invention can be used as a candidate vaccine for preventing and treating the equine influenza.