In vitro callus induction, regeneration and micropropagation of Solanum lycopersicum

Solanum lycopersicum belonging to family Solanaceae has a much lower sugar content than other edible fruits and is considered as vegetable for most culinary uses. Medicinally the plant is used as mild aperients, blood purifier, cholagogue and digestive. The plant is used in homoeopathy for treating rheumatic conditions, colds, chills, digestive disorders, diabetes, obesity, leucorrhoea, metrorrhagia. It promotes flow of bile; mildly laxative, especially when taken raw. Tomato is recommended for diabetics. It is a major dietary source of carotenoidlycopene. Tomato juice inhibits carcinogenic N-nitroso compound formation chiefly in the stomach. Tomato can decrease the risk condition such as cancer, osteoporosis and cardiovascular diseases (Bhowmik et. al.,2012). Callus induction and regeneration was previously reported by several workers( Gubis et.al,2004; Chen et.al.,1999; Devi et.al.,2008; Ishag et.al.,2009: Lima et.al., 2009; Chaudhury et.al,2010; Osman et.al., 2010 ).The present objective of research is to standardize the protocol for micropropagation of in vitro regenerated tomato plant.