Study of expression and effect factors on glucose transporter-1 of rabbit retinal Muller cells cultured in vitro

Objective To observe the different effect such as high coneentration of glucose and high concentration of insulin on GLUT1 of Rabbit Retinal Mailer Cell in vitro. Methods Rabbit retinal Muller cells were cultured in vitro with suspended constitution, which were divided as the following groups: common control group, high glucose group,insulin group, high glueose combined insulin group. Laser confocal microscope combined with immunocytochemical and fluorescence staining method to quantitatively analyze the expression condition of GLUT1. Results The expression of GLUT1 has been enhanced obviously by high glucose and high insulin,which locates mainly in the cytoplasm that near to the nucleus. Conclusion Rabbit retinal Mailer cells can express GLUTI,and the expression of GLUT1 can be reinforced by high glucose and high insulin. Key words: Glucose transporter type 1 ;  Neuroglia ;  Mailer cells ;