Development of pan-phlebovirus RT-PCR assay.

Alexander S. Klimentov,Alexander M. Butenko,Natalia V. Khutoretskaya,Elena Yu. Shustova,Victor F. Larichev,Olga V. Isaeva,Galina G. Karganova,Alexander N. Lukashev,Anatoly P. Gmyl

Development of pan-phlebovirus RT-PCR assay.

2016

Alexander S. Klimentov
Alexander M. Butenko
Natalia V. Khutoretskaya
Elena Yu. Shustova
Victor F. Larichev
Olga V. Isaeva
Galina G. Karganova
Alexander N. Lukashev
Anatoly P. Gmyl

This study reports the pan-phlebovirus assay capable of detecting both sandfly/mosquito- and tick-borne phleboviruses. Sensitivity and specificity of the assay was verified using a panel of arboviruses. The RT-PCR assay is simple and sensitive, and thus well suited for screening of field samples.

Keywords:

Molecular biology
Biology
Virology
Bunyaviridae Infections
Phlebovirus
Molecular diagnostic techniques
Sandfly
Real-time polymerase chain reaction
tick borne

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