Analysis of Transactivation Region of Elf-1 by Using a Yeast One-hybrid System

Chiharu Nishiyama,Kyoko Takahashi,Yasuyuki Ohtake,Toyokazu Yokota,Ko Okumura,Hideoki Ogawa,Chisei Ra

Analysis of Transactivation Region of Elf-1 by Using a Yeast One-hybrid System

2002

Chiharu Nishiyama
Kyoko Takahashi
Yasuyuki Ohtake
Toyokazu Yokota
Ko Okumura
Hideoki Ogawa
Chisei Ra

The transcriptional regulatory region of Elf-1 was analyzed by the combination of a yeast one-hybrid system and site-directed mutagenesis. This analysis enabled us to map an activation region between 85-175 of Elf-1.

Keywords:

Transcription factor
Transactivation
Mutagenesis
Hybrid system
Molecular biology
Yeast
Transcription (biology)
Genetics
Biology
regulatory region

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