A study of cyclic AMP-dependent protein phosphorylation in Schistocerca gregaria CNS: a comparison to that in mammalian CNS

Abstract 1. 1. The effect of cyclic AMP (10 μM) on the incorporation of 32 P into protein was studied in cell-free preparations of Schistocerca gregaria cerebral ganglia. 2. 2. Cyclic AMP-dependent phosphorylation of total protein was maximal after 60 sec. had a pH optimum of 7 to 8, was not affected by temperature (22–37°C) and had a K m of 77 μM ATP. 3. 3. Cyclic AMP increased the phosphorylation of total and specific protein in soluble fractions > synaptosomal > microsomal > crude membrane fractions. 4. 4. In a direct comparison of locust brain to rat cerebral cortex, cyclic AMP stimulated the increased phosphorylation of only three protein bands, whereas in identical fractions of locust brain the phosphorylation of at least 12 protein bands was observed.