Availability and development of an enzyme immunomicrosensor based on an ISFET for human immunoglobulins

Abstract A critical evaluation of the potentiometric response of an enzyme immuno-ISFET sensor has demonstrated that it is an effective, simple sensor for human immunoglobulin (IgG). The sensor was constructed using an immobilized human IgG membrane and an ISFET. The assay procedure involves the competitive immunochemical reaction of ureuse-labelled anti-human IgG with human IgG in samples and membrane-bound IgG and the electrochemical determination of membrane-bound urease activity. A linear relationship was obtained between the initial rate of response and the logarithm of IgG concentration from 0.1 to 2.0 mg ml −1 .