Determination of mitomycin C in human blood plasma and urine by high-performance differential pulse polarography

Abstract High-performance differential pulse polarography is used for determining the antitumor antibiotic mitomycin C in human blood plasma and urine. The limit of determination (2-ml samples) is 25 ng ml −1 when the substance is isolated by means of Amberlite XAD-2, and 200 ng mo −1 when samples are not pretreated. The method was applied in a pharmacokinetic experiment; no metabolites of mitomycin C were observed in urine or plasma.