Breeding and identification of SRC-3 knock-out mice.

Objective:To breed and identify steroid receptor coactivator-3(SRC-3) knock-out(SRC-3-/-) mice.Methods:Male SRC-3-/-mice were selected to hybridize with female heterozygote(SRC-3+/-) mice to acquire SRC-3+/-and SRC-3-/-baby mice.Genome DNA extracted from the murine tails was subjected to PCR for genotype identification.The total proteins of hepatic and spleen tissue were extracted to identify protein phenotype by Western blot.Results:More SRC-3-/-mice were breed successfully by hybridizing male SRC-3-/-mice with female heterozygote(SRC-3+/-) mice and their genotype were identified accurately by PCR.The expression level of SRC-3 in spleen tissue was significantly higher than that in hepatic tissue in SRC-3+/+ mice,but no expression of SRC-3 was observed in both hepatic and spleen tissue in SRC-3-/-mice.Conclusion:Hybridization of male gene knock-out mice and female heterozygote mice is an effective way to acquire considerable SRC-3-/-mice,and PCR is a simple and accurate method of identification of genotype.