沙田柚谷胱甘肽转移酶基因(CmGST)的生物信息学及表达分析 Bioinformatics and Expression Analysis of GST Gene Encoding Glutathione S-Transferase in Citrus maxima cv. Shatian Yu

利用高通量测序对沙田柚自交和异交花柱进行转录组测序，通过差异分析得到了沙田柚谷胱甘肽转移酶基因(CmGST)序列。CmGST全长为872 bp (GenBank登录号为KY612456)，开放阅读框为657 bp，编码219个氨基酸，推测蛋白质分子量为25.00 kDa，理论等电点pI为5.48。利用生物信息分析软件对CmGST基因进行同源性比对和系统进化分析表，结果显示该基因编码的氨基酸具有谷胱甘肽S转移酶典型的C端与 N端双结构域，属于GST Tau家族蛋白；与克莱门柚(Citrus maxima)的相似度约为98%。这些分析结果可为今后深入研究该蛋白的结构特征和功能提供参考。 In this paper, the transcriptome of the self-pollinated style and cross-pollinated style of Citrus maxima cv. Shatian Yu were sequences by high-throughput sequencing technology. The glutathione S-transferase gene (CmGST) sequence of Citrus maxima cv. Shatian Yu was obtained through differential analysis method. The fu11 length of CmGST gene was 872 bp (GenBank accession number: KY612456), and the ORF (Open Reading Frame) length was 657 bp, encoding a protein polypeptide of 219 amino acids with a predicted molecular weight of 25.00 kDa and pI of 5.48. Bioinformatics analysis showed that the CmGST protein contained the Tau GST-specific N-terminal domain (G site) and the C-terminal domain (H site). The homology analysis of amino acid sequence indicated that the glutathione S-transferase protein shared high homology with glutathione S- transferase protein of Citrus clementina (98%). This work provides a systemic sequence analysis of the glutathione S-transferase protein of Citrus maxima cv. Shatian Yu. It will provide the useful reference for further investigation of its structure and function.