Using Isolated Rat Kidney to Discover Kidney Origin Biomarkers in Urine

The use of targeted proteomics to identify urinary biomarkers of kidney disease in urine can avoid the interference of serum proteins. It may provide better sample throughput, higher sensitivity, and specificity. Knowing which urinary proteins to target is essential for targeted proteomics. In perfusates, there were proteins not found in normal human urine which may become biomarkers with zero background. There were proteins not found in normal human plasma which will not be influenced by other normal organs and will be kidney specific. When compared with existing candidate biomarkers, over 90 % of the kidney origin proteins in urine identified in this study have not been examined as candidate biomarkers of kidney diseases.