In vitro plant regeneration from unpollinated ovaries of Allium chinense

Abstract The present work was conducted to develop a simple and efficient protocol for in vitro shoot regeneration and plantlet formation from unpollinated ovary culture in Allium chinense . Ovaries that were excised from flowers 2–3 days before anthesis and cultured on induction medium with 9.05 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 8.88 μM benzylaminopurine (BAP) for 2 weeks gave the best organogenic response (56.32%). For shoot regeneration, the highest percent organogenic ovaries (64.53%) were obtained on B5 induction medium containing 9.05 μM 2,4-D and 8.88 μM BAP. Ovaries cultured on B5 containing 10.74 μM naphtaleneacetic acid (NAA) and 0.44 μM BAP exhibited maximum shoot regeneration (60.32%). Shoots elongated and produced normal plantlets when cultured on B5 medium supplemented with 10.74 μM NAA for 2 weeks. The plant regeneration procedure from the ovary may be an alternative for the improvement of A. chinense by genetic engineering.