EMBEDDING to stable isotope-labeled amino acid and its target protein, method for producing nmr structural analysis method and regioselective stable isotope-labeled fumaric acid and tartaric acid protein
2002
And at least one of the amino acids constituting the protein, the following labeling patterns; (A) 1 or 2 or more, leaving at least one hydrogen of the methylene hydrogen in the methylene group other are deuterated (b) prochiral gem- one hydrogen of the methyl groups in methyl completely the hydrogens of the methyl group in deuterated in that (c) prochiral methyl groups are partially deuterated in that (d) methyl group other leaving one hydrogen deuterated aromatic ring hydrogen moiety to provide a stable isotope-labeled amino acids, characterized in that it comprises at least one of which is deuterated. Using this stable isotope-labeled amino acids, deuterium substitution of the protein, the NMR sensitivity of the remaining hydrogen nuclei to achieve without impairing quick and reliable analysis of the NMR spectrum of the high molecular weight protein beyond previous limits, it is possible to achieve high-precision determination of the three-dimensional structure at the same time.
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