AS-183, a novel inhibitor of acyl-CoA : cholesterol acyltransferase produced by Scedosporium sp. SPC-15549

A novel compound, AS-183, which inhibits acyl-CoA:cholesterol acyltransferase (ACAT), was isolated from the culture broth of a fungus, Scedosporium sp. SPC-15549. AS-183 inhibited ACAT activity in an enzyme assay system using rabbit liver microsomes with an IC 50 value of 0.94 μM. AS-183 also inhibited cholesterol ester formation in HepG2, CaCo2, and THP-1 cells with IC 50 values of 18. 1, 25.5, and 34.5 μm, respectively