Acid–base properties of the reaction product of sialic acid with fluorogenic reagent, 1,2-diamino-4,5-methylenedioxybenzene (DMB)
2000
Abstract N -Acetylneuraminic acid (Neu5Ac) forms the highly fluorophoric quinoxalinone derivative ( Q ) when treated with 1,2-diamino-4,5-methylenedioxybenzene (DMB). Effects of protonation and deprotonation on the fluorescence of Q were examined at room temperature. The strong fluorescence was found to be caused by the neutral form Q but not the protonated form of its excited state [ Q ]* and at pH below 1 the emission was completely quenched. The deprotonated singlet form [ Q − ]* was a less efficient fluorescer than [ Q ]*.
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