The Role of Lysophospholipid Acyltransferases in the Golgi Complex
2016
: Determining the abundance of phospholipids and neutral lipids in cellular membranes is paramount to understanding their biological functions. Many lipid-modifying enzymes have yet to be characterized due to limitations in substrate-product measurements and purification of membrane-bound enzymes. The method described here uses radiolabeled phospholipid substrates and cell-purified organelles to quantify phospholipid metabolism using thin-layer chromatography. This assay has the benefits of being specific and adaptable for numerous applications and systems.
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