Проявление иммунокорригирующего эффекта криоконсервированных клеток фетальной печени разных сроков гестации в условиях развития экспериментальной модели реакции «Трансплантат против хозяина»*

In the research there has been estimated an immune correcting activity of cryopreserved and native fetal liver cells (FLCs) of different gestation terms in experimental model of local GVHR CIGVHR). IGVHR was induced in C57BI/6 mice by means of subcutaneous introduction of CBA/H lymph nodes CLN) cells into the hindpaw pad. In 24 hrs after initiation of IGVHR the mcie were intravenously injected with either native CnFLCs) or cryopreserved FLCs (cFLCs) of the 14th and 18th gestation days of CBA mice. To the 5th day after initiation of pathology the following indices were estimated: GVHR index, content of T (FoxP3+, CD4+CD25+ cells) of LNs with flow cytometer FACS Calibur (Becton Dicknson, USA), the expression of tfg-beta gene by means of PCR; content of IL-2, IL-10, TNF-alpha in blood serum of animals by means of immune enzyme method with analyzer Stat Fax 2100 (USA). It has been established that during induction of IGVDR there are manifested the signs characteristics for the pathologies of immune genesis. Under development of GCH, FLCs minimize clinical signs of pathologies and intensity of development of immune inflammatory reaction. Therapeutic activity of FLCs in greater extent correlated to the content in LNs of F0XP3+ cells and expression of tgf-beta gene, but not to the content of CD4+CD25+ cells and rate of their fluorescence. With the increasing of gestation terms from 14 post-coital days to 18 ones immune correcting activity of FLCs reduced. However after cryopreservation the FLCs-18 gained immune correcting activity of nFLC-14.