BIOTIN-AVIDIN AMPLIFIED ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR THE MEASUREMENT OF CANINE SERUM IGA, IGG AND IGM

Abstract An amplified capture enzyme-linked immunosorbent assay (ELISA) has been developed by the use of the biotin-avidin detection system, for the measurement of canine plasma immunoglobulins (Ig) A, G and M. Test responses of dilutions of both the Ig standards and test plasma samples were consistently linear (r >0·987) for the three Ig classes. The within-assay variation was 3·53 per cent IgG, 5·84 per cent for IgM and 6·34 per cent for IgA. The analytical recoveries were 95 per cent for IgA, 97 per cent for IgG and 98 per cent for IgM. The lower detection limits of the assay were 38·4 ng ml −1 for IgG, 20·3 ng ml −1 for IgM and 41·2 ng ml −1 for IgA. The results indicate that this ELISA has a much higher sensitivity than the single radial immunodiffusion assay or the non-amplified ELISA for measurements of canine Igs, but has a comparable specificity and precision.