Effects of emdogain on human periodontal ligament cells in vitro

Objective: To investigate the effects of emdogain,enamel matrix derivative(EMD),on the proliferation,cell cycle,mineralization and ultrastructure of human periodontal ligament(PDL) cells in vitro.Methods: The influence of cell growth on PDL cells was evaluated by Cell Counting Kit-8(CCK-8) in the presence and absence of emdogain,after 1,3,and 5 d of culture.DNA synthesis and ultrastructure of PDL cells were observed by flow cytometry(FCM) and transmission electron microscopy(TEM) in the presence and absence of emdogain after 3 d of culture.The increasing of osteogenic capa-city was verified by the expression changes of osteogenic differentiation markers of bone sialoprotein(BSP) and osteopontin(OPN) in emdogain-treated PDL cells by immunohistochemicl staining.Results: Incubation of PDL cells with emdogain after 3 d significantly stimulated cell growth and DNA synthesis.Emdogain enhanced the osteogenic potential of PDL cells by high expression of osteogenic diffe-rentiation markers of BSP and OPN.Conclusion: The data indicate that Emdogain enhances cell prolife-ration and promotes differentiation of PDL cells,which contributes to periodontal tissue regeneration.