Disruption of Lipid Profile and Alteration of Hepatic Lipoprotein Metabolism Gene Expression in Anaemia-induced Rat
2019
Background and Objective: Iron metabolism in animals is altered by haemolytic anaemia induced by
phenylhydrazine (PHZ), however, its effects on lipid metabolism remains elusive. The aim of this study
was to examine the impact of anaemia on lipid profiles and lipoprotein metabolism gene expression in
rats. Materials and Methods: Fourteen adult male Wistar rats were randomly classified into normal
control and anaemia‐induced group (n = 7), respectively. Anaemia was induced in rats by daily
administration of PHZ at 10 mg kg–1 for 8 consecutive days, after which blood was collected and liver
excised. Lipid profiles of plasma and liver were determined spectrophotometrically while the expression
of genes associated with lipid and lipoprotein metabolism was assayed by reverse transcriptase
polymerase chain reaction. Results: The induced‐anaemia resulted in hypotriglyceridemia and
hypophospholipidosis, with concurrent hypercholesteromia compared to control, respectively. Liver
triglycerides, phospholipids, cholesterol were observed to be up‐regulated. Anaemic rats showed a
significant (p<0. 05) up‐regulation of the relative expression of hepatic lecithin‐cholesterol
acyltransferase (Lcat), paraoxonase‐1 (Pon‐1), aryl hydrocarbon receptor (Ahr), 3‐hydroxy‐3‐
methylglutaryl‐CoA reductase (Hmgcr) and down‐regulation of Scavenger Receptor Class B Type I
(Scarb1). Conclusion: The induced‐anaemia alter the expression of lipoprotein metabolizing genes which
might be the underlying mechanism of anaemia to disrupt lipid metabolism.
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