Study on Anti-Osteoporosis of Compound Pearl Protein Polypeptide

Compound pearl protein polypeptide (CPPP) was investigated by MTT method as well as detecting alkaline phosphatase (ALP) and mineralized nodes in osteoblasts from SD rats. In vivo the rat models of osteoporosis were induced by ovariectomy and treated with three doses of CPPP (5.0mg.kg-1.d-1, 20.0mg.kg-1.d-1, and 80.0mg.kg-1.d-1). The serum levels of estradiol (E2), osteocalcin (BGP), transforming growth factors (TGF-β1) and ALK, together with the bone mineral density (BMD) and the contents of calcium (Ca) and phosphorus (P) in 24h urine, were detected. The results show that CPPP can enhance cell proliferation, ALK activity and the number of mineralized nodes in rats osteoblasts. CPPP significantly increase the serum level of E2, TGF-β1, and BMD, and decrease the serum level of ALP, BGP and the contents of Ca, P in 24h urine. So, CPPP can increase bone density, and thus it is conductive to osteoporosis prevention and treatment of the elderly.