[Methylation status of IEX-1 gene promotor CpG island in malignant hematopoietic cell lines].

This study was to purposed to explore the methylation status changes of IEX-1 gene promoler CpG island and its relevance with occurrence of hematologic malignancies.The methylation status of IEX-1 gene promoter CpG island in 9 NB4,HL-60 U937,Raji,CA46,Jurkat,K562,CEM and Molt4 hematologic malignant cell lines was detected by using methylation-specific PCR,the methylation status of IEX-1 gene promoter CpG island in normal peripheral blood mononuclear cells treated by M.sssI enzyme and the methylation status of IEX-1 gene promoter CpG island in normal peripheral blood mononuclear cells untreated were used as positive and negative controls respectively.The results showed that the hypermethylation of IEX-1 gene promoter CpG islands was detected in NB4,Molt4 and Raji cell lines,as well as in normal peripheral blood mononcelear cells treated by M.sssl enzyme;the partial methylation st atus was found in CA46,CEM,U937,K562,HL-60 and Jurkat cell lines;the unmethylation status was observed in untreated normal peripheral blood mononcelear cells.It is concluded that the changes of methylation status of gene IEX-1 promoter CpG island correlates with hematologic manignancies to a certain extent.