Isolation and characterization of rice lesion mimic mutants from a T-DNA tagged population

A rice (Oryza sativa L. ssp. japonica cv. Nipponbare) T-DNA tagged population consisting of about 7000 individual lines was generated and screened for rice lesion mimic mutants in the Tl generation. Ten lines were found to develop spontaneous lesions in the absence of pathogen infection and displayed distinct lesion phenotypes. These mutants were tentatively designated as lml–1m10 (for lesion mimic), respectively. Lesion formation of lm mutants was developmentally regulated, and al1 the mutants showed stunted growth and reduced fertility. Genetic analysis demonstrated that all the mutations were recessive, and five partially fertile mutants (lm4–lm8) were derived from different loci. Mimic lesions occurring on the leaves of lm mutants resulted from cell death as revealed by trypan blue staining. Six of them (lm3–lm8) exhibited enhanced resistance to five bacterial blight isolates, indicating their wide spectrum resistance to this pathogen. These results imply that some lesion mimic mutations of rice might...