Effect of multi-reflection on analysis of acoustic impedance of cultured cells

To understand physiologic state in diseased liver e.g. non-alcoholic steatohepatitis, this study aims to analyze the acoustic impedance (Z) of hepatic cells using a scanning acoustic microscopy (SAM). Ultrasound scattering sources in cells, however, cause the multi-reflection and prevent from analyzing the precious value of Z although it provides the useful information of structure in cells (M. N. Fadhel et al. 2015). A similar effect also occurs in case of a part of cells apart from substrate. As the fundamental study, this study discusses the effect of multi-reflection on the analysis of the Z of cultured cells.