Studies on antitumor effects and acute toxicity of anuoning emulsion

OBJECTIVE To investigate the antitumor effect and acute toxicity of anuoning emulsion in mice.METHODS The models transplanted tumor in mice and nude mice were used.Blliss method was used to calculate the lethal dose of 50% (LD 50 ) of anuoning emulsion in mice.RESULTS Under the doses of 4,8 and 16 mg·kg -1 anuoning emulsion intragstic (ig),qd×10 d,the average tumor inhibition rates were 39.2%,46.9% and 55.7% respectively ( P 0.01),against mice tumor HepS.The average tumor inhibition rates were 34.6%,47.3% and 54.4% ( P 0.01) against S-180 sarcoma in mice.In L 2 tumor,the mean inhibition rates were 37.5%,45.9%,and 56.5% ( P 0.05～0.01),respectively.Under the doses of anuoning emulsion 7.5,15,30 and 60 μg·kg -1 ,intraperitoneal injection (ip) qd×10 d to mice tumor HepS,the mean inhibition rates were 31.0%,42.9%,50.2% and 62.4% ( P 0.05～0.01),respectively.But In S-180 sarcoma the mean inhibition rates were 23.6%,39.4%,50.9% and 61.2% ( P 0.05～0.01),respectively.Under the doses of anuoning emulsion 15,30 and 60 μg·kg -1 ,ip×6/w×4 w,the inhibitory rates were 32.0% ,50.5% and 60.2% ( P 0.01) against xenograft tumor of human liver carcinoma (Bel-7402) in nude mice.Under the same doses,ip×6/w×3 w,the inhibition rates were 30.3%,41.9% and 56.1%,respectively,against xenograft tumor of human lung adenocarcinoma (GLC-82) in nude mice.The results showed that anuoning emulsion possessed antitumor effect against above-mentioned 5 tumors in mice and nude mice after ip and ig administration.In single intragastic (ig) management to mice,LD 50 and its 95% confidence interval was 74.75 (58.669～95.26) mg·kg -1 .In single ip management to mice,LD 50 of anuoning emulsion was 1.12 ( 1.01～ 1.24) mg·kg -1 .In single intravenous (iv) administration LD 50 was 1.81 (1.61～2.02) mg·kg -1 .CONCLUSION The experiment results indicated that anuoning emulsion possessed obvious antitumor effects against various transplanted tumor in mice and nude mice.