Effect ofdiphenylhydantoin sodium polybutylcyanoacrylate nanoparticles on rat models of epilepsy

：Objective To evaluatethe effects of diphenylhydantoin sodiumpolybutylcyanoacrylate nanoparticles (DPH-PBCA-NPs)and DPH-PBCA-NPs modified with Tween-80on rat models of epilepsy, and investigate theadvantage of nanoparticle as the drug delivery system.Methods The rat models of acuteepilepsy induced by lithium pilocarpine were established and randomly divided into 5groups: group Ⅰ (performinginjection of DPH-PBCA-NPs modified with Tween-80), group Ⅱ (performing injection of DPH-PBCA-NPs), group Ⅲ (performing injection ofdiphenylhydantoin sodium), group Ⅳ (performing injection of PBCA-NPs) and group Ⅴ(performing injection of physiologicalsaline). The changes of electroencephalogram (EEG) manifestations of these rats wereobserved by using video-EEG monitoring; and their behavioral changes were notedtoo.Results The lithium pilocarpine induced rat models of acute epilepsy were successfullyestablished and their status epilepticus were confirmed by EEG and their behaviors. Theeffective rate of DPH-PBCA-NPs modified with Tween-80 and DPH-PBCA-NPs was 91.67% and54.55%, respectively;the effective rate of rats in group Ⅲ was 50%, and the effective rate of rats in group Ⅳ and Ⅴ was 0%;DPH-PBCA-NPs modified with Tween-80 enjoyed abetter effect than DPH-PBCA-NPs and DPH (P＜0.05). Conclusion DPH-PBCA-NPs and DPH-PBCA-NPs modified withTween-80 can be used to improve the behaviors of rats with acute epilepsy and modify theresults of EEG of these rats.Nanoparticles as drug delivery system can help the drugshaving their effects much quickly and effectively. Key words: Epilepsy;  Nanoparticle;  Diphenylhydantoin sodium;  Poly n-butylcyanoacrylate