Anthocyanins from new red leaf tea 'Sunrouge'.

New red leaf tea cultivar ‘Sunrouge’ (Camellia taliensis × Camellia sinensis), for which an application for registration was made in 2009, is an anthocyanin-rich tea. The anthocyanin content of ‘Sunrouge’ was the highest among 4 tea cultivars, and was 8.4 times higher than that of ‘Yabukita’. We purified and isolated 6 anthocyanins from ‘Sunrouge’ by chromatography, and identified them by LC/MS/MS and NMR analysis. As a result, the four anthocyanins were identified as delphinidin-3-O-β-d-(6-(E)-p-coumaroyl)galactopyranoside (2), delphinidin-3-O-β-d-(6-(E)-p-coumaroyl)glucopyranoside (3), cyanidin-3-O-β-d-(6-(E)-p-coumaroyl)galactopyranoside (4), and cyanidin-3-O-β-d-(6-(E)-p-coumaroyl)glucopyranoside (5), and the other two were estimated as delphinidin-(Z)-p-coumaroylgalactopyranoside (1), petunidin-(E)-p-coumaroylgalactopyranoside (6). Compound 3 was found in tea for the first time. In general, anthocyanins have various bioactivities, including relieving eyestrain and antioxidative effects, so it is expe...