CAPILLARY ZONE ELECTROPHORESIS ANALYSIS OF ANTI-EPILEPTIC DRUGS

This paper describes the development of a simple, reliable, reproducible, and fast method, based on capillary zone electrophoresis with diode array detection, for the separation and determination of two antiepileptic drugs. Phenytoin (DPH) and phenobarbital (PB) were separated in 4 min by using a carrier containing glycine 50 mM, pH 10.5. The limit of detection was 0.1μg mL−1 for both compounds. Satisfactory reproducibility was obtained for migration times (<1.4%) and corrected peak areas (<6.9%) in the analysis of pharmaceutical formulations. The average recoveries obtained, which ranged between 100 and 108%, testify to the accuracy of the proposed method.