In-vitro experimental studies of selected biopesticides & their effect on selected plant pathogens

The present work will focus on the importance of pl ant base biopreparations and their antifungal and a ntibacterial activity. The plants were selected for preparation of Extracts (biopreparations) to test their broad s pectrum of resistance against for the selected plant pathogens as per the literature sources (Anonymous, 1948-76, Dhar., M.L., M.M.Dhar, B.N.Dhawan, B.N.Mehrotra and C.Ray. 1968 , Chopra. R. N., Nayar. S. L. and Chopra. I. C. 1986 , Bharath Kumar., R.2000.) There are about 15 indigenous plants were selected which are having diversifi ed uses like medicinal, economic, timber and fibre yielding spec ies like , Ocimum sanctum, Azadarichta indica, Thespesia populnea, Sida cordifolia, Pisonea alba, Nerium ind icum, Ficus religiosa, Ricinus communis, Colotropis gigantea, Eucalyptus globulous Achyranthes aspera, Catharanthus roseus, Albizia le bbeck, Typha angustifolia and Agave americana. viz.of Guntur region (Vadlamudi, Tenali etc.). The Antifungal activity was tested against 2 pathogens , which are very much prone to cause severe damage to the commercial crops viz., Solanum melongena and Gossypium herbacium and the anti bacterial activity was tested against the 2 pathogens, which causes considerable amount of yield loss against the crops like Lycorpersicum esculentum and Citrus limonium. A total number of 90 plant extracts were prepared as an individual of 45 ethanol, methanol and acetone solvent extracts bel ongs to the 15 individual plant species (plant parts ‐leaf, St.b e tc.) and another set of 45 plant extracts were prep ared in combination ethanol, methanol and acetone solvents. All these plant extracts were subjected against t heir antibacterial and anti fungal screening analysis, o ut of these 90 plant extracts (both individual and in combination) 44 plant extracts have been expressed the cognizabl e zone of expression i.e. 25 mm > 30 mm inhibition. Therefore these combinations were suggested for further analy sis of producing a novel broad spectrum of biopesti cides for crop protection.