Smart Hydrogel Particles: Biomarker Harvesting: One-Step Affinity Purification, Size Exclusion, and Protection against Degradation

A method for biomarker harvesting were an affinity bait molecule is introduced into N- isoproplacrylamide to produce a particle that will perform at least three independent functions within minutes. This incudes a molecular size sieving, affinity capture of all solution phase target molecules, and complete protection of harvested proteins from enzymatic degradation, the captured analytes can then be readily electroeluted for analysis.