Simultaneous analysis of 5′-deoxy-5-fluororidine and 5-fluorouracil in plasma by analytical isotachophoresis

Abstract Analytical isotachophoresis was used for the determination of 5-fluorouracil and 5′-deoxy-5-fluorouridine in plasma. The inclusion of spacers in the system greatly improved the separation and quantitation. The method can be employed for simultaneous measurements of different fluorinated pyrimidines used in clinical practice.