Plasma lipid analysis by hydrophilic interaction liquid chromatography coupled with electrospray ionization tandem mass spectrometry
2015
A novel method for the analysis of endogenous lipids and related compounds was developed employing hydrophilic interaction liquid chromatography with electrospray ionization tandem mass spectrometry. A hydrophilic interaction liquid chromatography with carbamoyl stationary phase achieved clear separation of phosphatidylcholine, lysophosphatidylcholine, sphingomyelin, ceramide, and mono-hexsosyl ceramide groups with good peak area repeatability (RSD% 0.99). The established method was applied to human plasma assays and a total of 117 endogenous lipids were successfully detected and reproducibly identified. In addition, we investigated the simultaneous detection of small polar metabolites such as amino and organic acids co-existing in the same biological samples processed in a single analytical run with lipids. Our results show that hydrophilic interaction liquid chromatography is a useful tool for human plasma lipidome analysis and offers more comprehensive metabolome coverage.
Keywords:
- Analytical chemistry
- Thermoresponsive polymers in chromatography
- Liquid chromatography–mass spectrometry
- Hydrophilic interaction chromatography
- Electrospray ionization
- Metabolome
- Direct electron ionization liquid chromatography–mass spectrometry interface
- High-performance liquid chromatography
- Chromatography
- Chemistry
- Reversed-phase chromatography
- Tandem mass spectrometry
- Lipidome
- Correction
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