조직/세포배양 : 자색고구마의 잎 조직배양을 통한 식물체 재생

This study was carried out to establish a regeneration system from leaf explant of purple sweetpotato(Ipomoea batatas L.) The optimal concentrations of plant growth regulators for callus induction and shoot formation were determined. The optimal combination for callus formation was 1M 2,4-D 5M BM, and highest yield of embryogenic calli were observed on Murashige and Skoog basal medium containing 0.5M 2,4-D under light condition after 4weeks of culture. Embryogenec callus was subcultured on medium supplemented with 5M ABA for 4 days. Subsequently, regeneration of adventitious shoots occurred when these embryogenic calli were transferred onto medium with 3∼6M gibberellic acid. Regenerated shoots were developed into normal plantlets.