Development of a particle concentration fluorescence immunoassay for the quantitative determination of IgG in bovine milk

A rapid solid-phase particle concentration fluorescence immunoassay (PCFIA) was developed to quantitate the total IgG concentration in bovine milk. The assay is based on agglutination of antibody covalently bound to carboxyl-polystyrene particles with the antigen, which is in turn detected by a fluorescent labeled antibody by epifluorometry. PCFIA is simple to perform, sensitive (5 ng of IgG/mL), accurate, repeatable, and faster than most immunoassays