Study on ultrasonic wave extraction of total flavonoid from flower bud of Chrysanthemum indicum and its antimicrobial effect bacteriostasis experiment.

Experiment was conducted on total flavonoid from flower bud of Chrysanthemum indicum with water as solvent by ultrasonic wave extraction.Single factor experiment and the orthogonal experiment were used to determine the optimal processing technology.Determination of MIC of 7 common pollutant bacterium was by disc diffusion method.The result indicated that optimal ultrasonic wave extraction technology of total flovonoid was as follows: temperature of 70℃,ratio of material to liquid 1:120(g:mL),duration of 40 minutes,yield of total flavonoids 16.96 %.The extract of flower bud of Ch.indicum had antimicrobial activity against Escherichia coli,Bacillus subtilis,Staphylococcus aureus,Saccharomyces cerevisiae,Aspergillus flavus and Penicillium chrysogenum,and it had bactericidal effect on E.coli,B.subtilis,S.aureus,S.cerevisiae.The extracts had better antimicrobial and bactericidal activity against germs than molds.