Giant unilamellar vesicles - a perfect tool to visualize phase separation and lipid rafts in model systems.

- perature. This mini-review presents information about the methods of GUV production and their usage. Particularly, the use of GUVs in studying lipid phase separation and the appearance and behavior of lipid domains (rafts) in membranes is discussed but also other examples of GUVs use in membrane research are given. The experience of the authors in setting up the GUV-form - ing equipment and production of GUVs is also presented.