Fraksi aktif antijamur Candida albicans dari ekstrak metabolik bunga kemilan (matricaria chamomilla L)(Anti-fungal active fraction to Candinda albicans from methabolic extract of camilen flower (Matricaria chamomilla, L)

The flowers of kamilen (Matricaria chamomilla, L) has been used as antifungal in Indonesian traditional medicines. Previous study showed that the extract of flowers inhibited Candida albicans activity. In order to determine the active fraction, the bioactive-guided fractionation method was used. The air-dried flowers was extracted from methanol. Methanolic extracts was fractionated by using n-hexane, chloroform and ethyl acetate, n-Hexane fraction was seoarated by vacuum liquid chromatography (VLC) by using silica gel H60 with mobile phase n-heksan; ethyl acetate and chloroform: methanol by gradient polarity. VCL results fraction was separated by preparative thin layer chromatography with silica get GF254 and mobile phase n-hexane: chloroform (1:1). The anti-fungal activities of Candida albicans was done by difuse method. Based on the TLC appearance, nine fractions were obtained (FK 1-9) from VLC and FK-3 was the most active fraction in inhibiting Candida albicans. The result from preparative TLC of FK-3 was 5 subfractions (PP 1-5). Subfraction FP-3 and FP-5 were most active, that had inhibiting radical zone diameter 18 mm in 3% consentration.