Analysis of Stability of Transcription System in Octane Media by Molecular Dynamics Simulation
2019
Enzymatic catalysis in nonaqueous environments has many advantages over that in aqueous solution. However, structural stability and flexibility of enzymes restrict their applications in nonaqueous environments. It was proposed that nonaqueous media decrease the flexibility of enzymes, resulting in their reduced activity. There is little detailed information to clarify the impact of nonaqueous solvents on the structure and flexibility of enzymes at molecular level. In this work, by using the molecular dynamics simulation technology, we placed an entire transcription system in organic solvent to investigate the effects of nonpolar solvent on an enzyme. A small transcription machine, T7 RNA polymerase (T7 RNAP), was used as model enzyme to conduct this study. We constructed two models with the T7 RNAP complex solvated in organic solvent. The first one is to solvate the T7 RNAP complex in pure octane directly. Our observation shows that T7 RNAP suffers serious structural deterioration in octane, especially in...
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
38
References
0
Citations
NaN
KQI