Biosynthesis of ergosta-6(7),8(14),22(23)-trien-3β-ol by Gibberella fujikuroi: its importance to Ergosterol's metabolic pathway

A series of ergostatrienes: ergosta-8(9),14(15),22(23)-trien-3β-ol (1), ergosta-5(6),8(9),22(23)-trien-3β-ol (2), ergosta-5(6),7(8),22(23)-trien-3β-ol(3), and ergosta-6(7),8(14),22(23)-trien-3β-ol (4), have been isolated and characterized by chromatographic and spectral methods from mycelia of Gibberella fujikuroi and through appropriate feeding and trapping experiments the sequence (1)→(2)→(3) has been demonstrated with (4) being an unmetabolized end product of biosynthesis.