Positive effect of autologous platelet rich plasma on Saanen buck semen cryopreservation in non-breeding season.

Abstract The objective of the current study was to evaluate the effect of autologous platelet-rich plasma (PRP) addition into soybean lecithin based extender on buck semen at post-thaw. Semen samples were collected from eight Saanen buck, and each semen sample was split into four equal aliquots and diluted with different concentrations of PRP supplemented extenders [no PRP (control), 0.5x107/ml PRP, 1x107/ml PRP, or 2x107/ml PRP]. Motility, plasma membrane functional integrity, acrosome integrity, mitochondrial membrane potential, DNA integrity and malondialdehyde concentrations (MDA) were measured and analyzed at post-thaw. The results showed that 2x107/ml PRP group had a positive effect on motility (62.41±4.24), membrane functional integrity (71.11±2.90), mitochondrial membrane potential (69.70±1.99), DNA integrity (7.22±0.93) and MDA levels (2.56±0.73) at post-thaw (P