Distribution characteristics of alleles of classical SLA-I and II genes and bioinformatic analysis of novel alleles in Guizhou miniature pigs.

This article has systematically elucidated the alleles distribution characteristics of classical SLA class I and II genes, and analyzed novel alleles in Guizhou miniature pigs via bioinformatics. The frequency of twenty five allele sequences from seven SLA - I and II genes previously derived in this population were calculated. There existed five alleles in SLA-2, four alleles in SLA-1, three in QA, QB1 locus, and two in SLA-RA, RB1, respectively. The dominant alleles were evident; every locus had 1 or 2 dominant alleles, one in SLA-3, two in SLA-1 two in QA, QB1: SLA-RA and SLA-RB1 loci have only two alleles. Their distribution is relatively uniform. The comparison between novel SLA class I and II gene sequences and reference sequences showed highly polymorphic region of SLA-I focus on the α1 and α2 areas of amino acid sequence and the polymorphic region of SLA-II mainly expressed in α1 area. The novel alleles and the referenced sequences showed more than 92% homology in amino acid level. The highest amino acid homology percentage of novel alleles and HLA alleles in NCBI were more than 70%. Novel alleles have some functional sites of HLA. According to the analysis, Guizhou miniature pigs have obvious SLA characteristics, it could be the evidences for further haplotypes selection, pig breeding and xenotransplantation.