Organogenesis from the cultures of Nothapodytes foetida (Wight) Sleumer raised on TDZ supplemented media

Mature embryos of Nothapodytes foetida (Wight) Sleumer were inoculated on Murashige and Skoog (MS), modified MS (MMS) and Phillips and Collins (L2) media supplemented with TDZ either alone or with BAP and L-glutamine. Both shoot proliferation from the apical region of shoots and differentiation of shoots from the callus were observed from the cultures depending on the type of medium and the hormone combination. Proliferation of shoots from the apical region of shoots and also organogenesis from the callus, formed at the transition zone of primary root and hypocotyl of the young seedling, was obtained on L2+TDZ (0.44 µM)+BAP (2.22 µM)+L-glutamine (0.03 mM). Regeneration from the callus cultures was reported for the first time. The dual role of TDZ in promoting proliferation of shoots and shoot differentiation from the callus, along with beneficial effects of cytokinin and L-glutamine on the morphogenesis, was discussed.