Detection of pim-1 mRNA in prostate cancer diagnosis.

Background Pim-1 plays an important role in the apoptosis,proliferation,differentiation of cancer cells and progression of cancer.In this study we detected the expression of pim-1 mRNA in normal prostate,benign prostatic hyperplasia (BPH),and prostate cancer(PCa)and explored its diagnostic value for PCa. Methods The prostate tissues were collected from 23 patients with PCa,37 patients with BPH,and 3 healthy volunteers.Pim-1 mRNA expression levels in these samples were determined by the quantitative real-time PCR (QRT-PCR).The differences of expression were calculated based on a standard curve. Results The ratio of pim-1 mRNA to β-actin in the normal prostate,BPH,and PCa were 1.05±0.04,2.57±0.74 and 4.45±0.63,respectively.The differences among PCa,BPH and NT were significant(P0.05,respectively). Conclusion Detecting pim-1 mRNA expression by QRT-PCR provides a reliable metric for the diagnosis of PCa. Chin Med J 2007;120(17):1491-1493