Highly Sensitive Method for Immunohistochemical Detection of Poly(ADP-Ribose) in Various Animal Species

Poly(ADP-ribose) has been expected to play some regulatory roles in nuclear functions. However, its amount and distribution in vivo have not yet been fully elucidated. An immunohistochemical method should be useful for the study of the natural occurrence of poly(ADP-ribose) at the cellular level. We have developed a highly sensitive immunohistochemical method for detecting poly(ADP-ribose) in conventional histological sections (1, 2).