STUDIES ON THE RELEASE OF ANTIBODY FROM SURVIVING TISSUE SLICES
1958
The interaction between tissue slices and surviving medium (serum) in some aspects such as electrophoretic pattern of serum protein and titer of hemagglutinin was investigated. Liver and spleen slices from normal and immunized rabbit were survived in Warburg-type flasks at 38.5°C, under an atmosphere of 95% O2, 5% CO2, for 6 hours. Following results were obtained. The release of the antibody of the immunized tissue slices survived in normal serum was observed. 1) The rate of release of protein and non-protein nitrogen from normal and immunized liver and spleen slices were not different from each other. However, release of antibody from immunized tissue slices was markedly increased. 2) The release of beta-and gamma-globulin from liver slices was demonstrated and the rate of release of gamma-globulin was accerelated by immunization. The same results, however, was not obtained in spleen slices. 3) The release of serum protein (beta- and gamma-globulin) and antibody from tissue slices were modified by immunization. And the difference between liver and spleen was observed. From these results, it was revealed that the protein metabolism of tissue in vitro was influenced by immunization and liver and spleen slices showed different reaction in vitro by immunization.
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