Flow Cytometric Characterization of Lymphocyte Subpopulations in the Korean Bovine

Characterization of immune cell subpopulations in the bovine was performed using a direct immunofluorescence technique adaptable for routine and repeated monitoring. This whole blood procedure is faster and requires less volume than conventional density gradient isolation methods. Low inter-and intra-animal variations were seen in hematology parameters and in CD4, CD8 and CD2 lymphocyte subtypes. CD4 values were 30% of lymphocytes in male and 32% in females. Thriteen percent were CD8 in males and 13% in females. CD4: CD8 ratios were approximately two in both sexes. Fiffty three percent were CD2 in males and 54% in femals. The mean RBC counts of peripheral blood were 7.20×10?/㎣ for male cattle and 6.36×10?/㎣ for females. The mean WBC counts were 8.09×10³/㎣ for males and 7.09×10³/㎣ for females. The percent of lymphocytes(63-65%) was higher than the percent of neutrophils(17-18%), the percent of eosinophils(1l-15%), te percent of monocytes(4-5%), and percent of basophiles(1%).