HPLC of carbohydrates with cation-exchange silica and resin-based stationary phases

Abstract Chromatographic analysis of carbohydrates is frequently performed on ion-exchange stationary phases. The development of new pressure-stable ion-exchange materials with small particle size and narrow size distribution enables improvements in column efficiency and reduced retention times. This chapter aims to provide an overview of various stationary phases in cation-exchange chromatography available for the separation of carbohydrates. Interaction of carbohydrates with the stationary phase and related separation mechanisms are discussed.