Light as a substitute for glutaminic acid oxidase and fluorimetric determination of glutaminic acid

Under the irradiation of UV-light, photochemical reaction of glutaminic acid occur and produces H2O2 and HN3 in weak alkaline medium with acetone. This reaction is equal in value for enzymatic reaction of glutaminic acid in the presence of glutaminic acid oxidase. Then, using hemin as catalyst, H2O2 produced by photochemical reaction oxidase p-hydroxyphenyl acetic acid (HPA) and causes fluorescence reaction coupled of it. Based on this result, a new fluorimetric method was developed for indirect determination of glutaminic acid.